What are the advanced practices for improving accuracy in genomic sequencing

Advanced practices for improving accuracy in genomic sequencing include using high-fidelity polymerases, optimizing library preparation protocols, and employing hybrid sequencing technologies that combine short and long-read sequences. High-fidelity polymerases reduce errors during amplification, ensuring more accurate sequencing results. Optimizing library preparation involves selecting the right kits and protocols to minimize biases and improve the quality of input DNA or RNA. Hybrid sequencing technologies, such as combining Illumina’s short-read accuracy with Oxford Nanopore’s long-read capabilities, provide comprehensive and accurate sequencing by leveraging the strengths of both methods. Additionally, implementing rigorous quality control checkpoints throughout the sequencing process, including the use of control samples and repeated runs, can further enhance accuracy. Finally, bioinformatics tools that employ machine learning algorithms can help correct sequencing errors and improve data interpretation. Source: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6604543/, Source: https://www.nature.com/articles/s41587-019-0209-z Key Takeaway: Use high-fidelity polymerases, hybrid technologies, and robust bioinformatics to enhance sequencing accuracy.